Journal: Cell Reports

Article Title: CBP-IDRs regulate acetylation and gene expression

doi: 10.1016/j.celrep.2026.117109

Figure Lengend Snippet: Sequence properties of CBP-IDRs (A) Sequence properties of CBP WT , CBP CFID , CBP IDR6 , and CBP IDR7 . Amino acids are grouped by property: polar (Q, N, S, T, G, H, C); proline (P); aliphatic (A, L, M, I, V); charged (E, D, R, K); aromatic (F, Y, W). Numbers indicate the fraction of amino acids with each property. (B) Linear amino acid composition ; green bar highlights 18× polyQ. (C) Comparison of the 12 most-enriched or -depleted sequence parameters (relative to all human IDR proteins) in CBP WT and shuffle mutants, using NARDINI+. , Features from main text are highlighted in bold. (D–F) Effect of shuffled IDRs on CBP condensates. (D) Confocal microscopy; scale bars, 10 μm. (E) Number of condensates per nucleus. (F) IID; n = 3 biological replicates. p values by Kruskal-Wallis test. (G) PLAAC analysis showing CBP WT , CBP IDR6-shuffle , and CBP IDR7-shuffle . See also .

Article Snippet: Fully assembled CBP WT -GFP was sequenced by Sanger sequencing using sequential primers at 900bp intervals and confirmed using Whole Plasmid Sequencing (Eurofins) to check for correct assembly.

Techniques: Sequencing, Comparison, Confocal Microscopy

Journal: bioRxiv

Article Title: Modified meiosis in the tardigrade Hypsibius exemplaris maintains heterozygosity across the genome

doi: 10.64898/2026.03.11.711151

Figure Lengend Snippet: (a) Using BLAST+, the predicted Hypsibius exemplaris telomeric repeat sequence (GATGGGTTTT) from was aligned to the putative five-chromosome genome assembly generated using Hi-C data by the DNA Zoo Consortium. Shown are the total counts of alignments of this sequence per 1Mb bin. (b) Heterozygosity across the five chromosomes of Hypsibius exemplaris was calculated from single-tardigrade sequencing data generated by , and using the chromosome-level genome assembly above. Heterozygosity was calculated in 100kb bins as the percentage of heterozygous bases out of all sites that passed filtering cut-offs. Heterozygosity was calculated for each of the four individuals sequenced, and the mean across the four replicates (per bin) is shown here. Alt text: Plots showing how alignment of telomeric repeats (a) and heterozygosity (b) varies across the length of five chromosomes.

Article Snippet: Whole plasmid sequencing data for both alleles of each locus was analyzed using Benchling to count heterozygous sites and identify restriction enzymes that cut one of the two alleles only.

Techniques: Sequencing, Generated, Hi-C

Journal: bioRxiv

Article Title: Modified meiosis in the tardigrade Hypsibius exemplaris maintains heterozygosity across the genome

doi: 10.64898/2026.03.11.711151

Figure Lengend Snippet: (a) Genes carrying predicted high-impact heterozygous variants in H. exemplaris . Only variants detected in all three bulk tardigrade sequencing datasets and at least one individual tardigrade sequencing dataset are shown. Bars indicate the number of genes per variant consequence class. Numbers in parentheses indicate the total number of variants in each class, since in a few cases a single gene contains two high-impact variants. (b-c) Genes with nonsense or frameshift variants that affect ≥25% of the predicted protein sequence. For nonsense variants, gray bars indicate the remaining protein length and red bars indicate the truncated portion. For frameshift variants, gray bars indicate the portion of the protein encoded before the frameshift and blue bars indicate the portion of the predicted protein sequence that is downstream of the frameshift. Importantly, frameshift variants are likely to impact total protein length by disrupting downstream stop codons, so the blue bars do not represent the length of the variant allele protein product. Bolded Gene IDs are examples with especially high mRNA expression mentioned in the text. (d) Distribution of mRNA expression levels for genes with high-impact heterozygous variants compared to all genes in the genome. Histograms show the fraction of genes at each expression level following log10 transformation of mean transcript abundance (TPM). Expression values represent the mean across four RNA-seq replicates from . Alt text: Graphs showing gene and variant counts in different categories (a), predicted protein product lengths for select genes (b-c), and a histogram of gene expression levels (d).

Article Snippet: Whole plasmid sequencing data for both alleles of each locus was analyzed using Benchling to count heterozygous sites and identify restriction enzymes that cut one of the two alleles only.

Techniques: Sequencing, Variant Assay, Expressing, Transformation Assay, RNA Sequencing, Gene Expression